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| Turchetto-Zolet,Andreia Carina; Christoff,Ana Paula; Kulcheski,Franceli Rodrigues; Loss-Morais,Guilherme; Margis,Rogerio; Margis-Pinheiro,Marcia. |
| Abstract Since the first diacylglycerol acyltransferase (DGAT) gene was characterized in plants, a number of studies have focused on understanding the role of DGAT activity in plant triacylglycerol (TAG) biosynthesis. DGAT enzyme is essential in controlling TAGs synthesis and is encoded by different genes. DGAT1 and DGAT2 are the two major types of DGATs and have been well characterized in many plants. On the other hand, the DGAT3 and WS/DGAT have received less attention. In this study, we present the first general view of the presence of putative DGAT3 and WS/DGAT in several plant species and report on the diversity and evolution of these genes and its relationships with the two main DGAT genes (DGAT1 and DGAT2). According to our analyses DGAT1, DGAT2,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Triacylglycerol biosynthesis; DGAT; Phylogeny; Gene structure. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000400524 |
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| Wasko,Adriane P.; Severino,Fábio E.; Presti,Flávia T.; Poletto,Andréia B.; Martins,Cesar. |
| An alpha actin gene segment, isolated from Nile tilapia (Oreochromis niloticus), was characterized by nucleotide sequencing, predicted amino acid sequence and Southern blot hybridization. Genomic DNA amplification resulted in a 1063-bp fragment corresponding to a partial alpha-cardiac muscle actin gene containing exons 3 to 6. Southern blot analysis of the restriction-digested DNA revealed that the Nile tilapia genome contains multiple muscle actin isoforms. Although comparison of the nucleotide sequence, amino acid residues and exon-intron organization of the isolated actin gene with those of other vertebrates showed a high level of identity, diagnostic amino acid residues can still be correlated to distinct actin genes in fish species. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Actin gene; Amino acid residues; Gene structure; Nile tilapia; Nucleotide sequence; Oreochromis niloticus. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000600010 |
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